Allergen Details
| Allergen name: | Cup s 2 |
|---|---|
| Allergen source: |
Major taxonomic group: Plantae Pinopsida Order: Cupressales Species: Cupressus sempervirens (Common cypress) NCBI Taxomony ID: 13469 |
| Biochemical name: | Polygalacturonase |
| MW(SDS-PAGE): | 43 kDa |
| Allergenicity: | sera from 19 of 31 subjects with airway allergy in areas exposed to Common cypress (Curessus sempervirens) were positive to a 43 kDa protein based on immunoblots. The protein was characterized and demonstrated to be highly identical to Jun a 2 (~95% identity) and to Cha o 2 (80%). |
| Allergenicity reference: | PubMed:28439939 |
| Route of allergen exposure: | Airway |
| Created: | 2017-01-11 16:03:09 |
| Last Updated: | 2019-08-09 07:16:52 |
| Submitter Info: | |
| Name: | Pascal Ponset |
| Institution: | Institut Pasteur |
| City: | Paris, France |
| Email: | pascal.poncet@pasteur.fr |
| Submission Date: | 2016-12-09 |
Comments
Note that the Uniprot entry for the protein sequence shows a number of gaps between peptide segments, and BLAST to the nearest sequence suggests that there are many gaps. Thus the UniProt sequence C0HKB1 is likely to be wrong.
Table of Isoallergens
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| Isoallergen and variants | GenBank Nucleotide | GenBank Protein | UniProt | PDB |
|---|---|---|---|---|
| Cup s 2.0101 | C0HKB1 |
Ten reads of LC-MSMS produced 33 overlapping peptides with high identity match to a homologous protein from Cupressus arizonica and to Juniperus ashei, with >74% coverage. Edmand degradation was used to identify the N-terminus. That sequence was submitted to UniProt as C0HKB1, which is not yet posted. Note the IgE binding to this protein in Allergy 2017 72(11):1806 shows total inhibition with bromelain. In addition, the sequence entered here and UniProt indicates many gaps as this was LC/MSMS