WHO/IUIS Allergen Nomenclature Sub-Committee

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Allergen Details:

Allergen name: Act d 12
Allergen source: Major taxonomic group: Plantae Eudikotyledons
Order: Ericales
Species: Actinidia deliciosa (Green kiwi fruit)
NCBI Taxomony ID: 3627
Biochemical name:Cupin, 11S globulin
MW(SDS-PAGE):50,207.304 Da (mass spectrometry), 6 peptide sequences (C0HJF9, to be released upon publication)
Allergenicity:Sera from 39 of 55 kiwifruit allergic individuals had IgE that reacted with the purified natural Act d 12 in immunobolots and ELISA. Basophil activation tests of 3 of 4 patients with the purified nAct d 12 were positive. SPTs of 4 of 4 patients with the purified nAct d 12 were positive.
Allergenicity reference:PubMed:25041438PubMed:24990823
Route of allergen exposure:Food
Date Created:28-11-2013
Last Updated:2019-06-28 06:47:23
Submitter Info:
Name:Oscar Palomares
Institution:School of Chemistry, Complutense University of Madrid, Department of Biochemistry and Molecular Biology
City:Madrid, Spain
Submission Date:2013-11-28


Posted: 01 Mar 2016

The full sequence of kiwi Act d 12 was not determined in the Palomares submission. The six pepetide sequences provided are obtained from the allergenic protein purified from Actinidia deliciosa. These peptides match with the available cDNA sequence for an 11S globulin-like protein from Actinidia chinensis. The new allergen (Act d 12) has been identified, purified and characterized from Actinidia deliciosa. The order of peptides (partial sequence) are based on the known sequence of Actinidia chinensis 11S. 24 Feb 2016

Posted: 02 Oct 2017

The Uniprot entry seems to be a fusion of the N-terminal peptide plus a partial fragment of later location in the protein. It does not match. WHO/IUIS sent an inquiry to Dr. Palomares to confirm. It seems other sequences in the JACI paper provide good BLASTP matches to a protein identified in 2006 by others. Dr. Palomares has contacted UNIPROT. We have added the full-length sequence of Actinidia chinensis, which has one amino acid mismatch from peptides that Dr. Palomares entered. This is to make sure the full-length sequence is identified, even if the sequence is from golden kiwi.

Table of IsoAllergens Click +/- for additional information
Isoallergen and variants GenBank Nucleotide GenBank Protein UniProt PDB
Act d 12.0101  C0HJF9 
Act d 12.0102DQ251185.1ABB77213