Allergen Details:
| Allergen name: | Act d 12 |
|---|---|
| Allergen source: |
Major taxonomic group: Plantae Eudikotyledons Order: Ericales Species: Actinidia deliciosa (Green kiwi fruit) NCBI Taxomony ID: 3627 |
| Biochemical name: | Cupin, 11S globulin |
| MW(SDS-PAGE): | 50,207.304 Da (mass spectrometry), 6 peptide sequences (C0HJF9, to be released upon publication) |
| Allergenicity: | Sera from 39 of 55 kiwifruit allergic individuals had IgE that reacted with the purified natural Act d 12 in immunobolots and ELISA. Basophil activation tests of 3 of 4 patients with the purified nAct d 12 were positive. SPTs of 4 of 4 patients with the purified nAct d 12 were positive. |
| Allergenicity reference: | PubMed:25041438 PubMed:24990823 |
| Route of allergen exposure: | Food |
| Created: | 2014-02-14 12:00:00 |
| Last Updated: | 2019-06-28 06:47:23 |
| Submitter Info: | |
| Name: | Oscar Palomares |
| Institution: | School of Chemistry, Complutense University of Madrid, Department of Biochemistry and Molecular Biology |
| City: | Madrid, Spain |
| Email: | oscar.palomares@quim.ucm.es |
| Submission Date: | 2013-11-28 |
Comments
The Uniprot entry seems to be a fusion of the N-terminal peptide plus a partial fragment of later location in the protein. It does not match. WHO/IUIS sent an inquiry to Dr. Palomares to confirm. It seems other sequences in the JACI paper provide good BLASTP matches to a protein identified in 2006 by others. Dr. Palomares has contacted UNIPROT. We have added the full-length sequence of Actinidia chinensis, which has one amino acid mismatch from peptides that Dr. Palomares entered. This is to make sure the full-length sequence is identified, even if the sequence is from golden kiwi.
| Isoallergen and variants | GenBank Nucleotide | GenBank Protein | UniProt | PDB |
|---|---|---|---|---|
| Act d 12.0101 | C0HJF9 | |||
| Act d 12.0102 | DQ251185.1 | ABB77213 |
The full sequence of kiwi Act d 12 was not determined in the Palomares submission. The six pepetide sequences provided are obtained from the allergenic protein purified from Actinidia deliciosa. These peptides match with the available cDNA sequence for an 11S globulin-like protein from Actinidia chinensis. The new allergen (Act d 12) has been identified, purified and characterized from Actinidia deliciosa. The order of peptides (partial sequence) are based on the known sequence of Actinidia chinensis 11S. 24 Feb 2016